Evaluation of a New Culture-Based AtbFinder Test-System Employing a Novel Nutrient Medium for the Selection of Optimal Antibiotics for Critically Ill Patients with Polymicrobial Infections within 4 h

Here, we describe the validation of a new phenotypic culture-based AtbFinder method for rapid selection antibiotics in vitro using specimens with mono- and polybacterial infections. AtbFinder, which can be applied to any type non-blood tissue, does not require isolation pure bacterial cultures. The uses novel TGV medium that allows more growth Gram-positive Gram-negative monoisolates compared achieved conventional laboratory media, demonstrating overall sensitivity, specificity, PPV, NPV values 99.6%, 98.1%, 98.5%, 99.4%, respectively, after 4 h. For polymicrobial infections, utilized paradigm population response antibiotics, enabling form mixed microbial community selecting targeting only principal pathogen, but also those bacteria support their growth. allowed culturing diverse set from biospecimens, standard enabled, within h, accurate completely eliminated all cultivatable clinical samples. In conclusion, system may valuable tool improving antibiotic selection, targeted empirical therapy replacement, is especially important high-risk patients.